How do you approximate the pH based on transmittance and absorbance data?

In college bio lab we used a spectrometer to collect transmittance at certain wavelengths (400-650, at 50 intervals), and absorbance at those wavelengths, for solutuions that have a certain pH value. We also had two unknown solutions, and we have to approximate the pH value for those two solutions. How do I do... show more In college bio lab we used a spectrometer to collect transmittance at certain wavelengths (400-650, at 50 intervals), and absorbance at those wavelengths, for solutuions that have a certain pH value. We also had two unknown solutions, and we have to approximate the pH value for those two solutions. How do I do that? How is absorbance and transmittance related to pH? All the absorbance numbers are less than 1, and the transmittance numbers are less than 100.
1 answer 1